5. Separation by Chromatography
Chromatography is a technique of separating two (or more) dissolved solids which are present in a solution in very small quantities.There are many types of chromatography but the simplest form is the paper chromatography.So, we will study only paper chromatography in this class. By using paper chromatography, we can separate two (or more) different substances present in the same solution. This separation is based on the fact that though two (or more) substances are soluble in the same solvent (say, water) but their solubilities may be different. Some may be more soluble than the others. For example,black ink contains a number of dyes dissolved in water. These dyes have different solubilities in water.This fact is used to separate the dyes present in black ink.
It is most easy to perform chromatography experiments with the mixtures of coloured materials like "inks' and other 'dyes' because they separate to give coloured components which can be seen easily as coloured spots on the paper. A special type of paper called "chromatography paper' is used for carrying out separations by chromatography. For ordinary purposes, however, filter paper can also be used for paper chromatography experiments. We will now describe the process of separation of dissolved solids from a solution by paper chromatography by taking the example of black ink. Black ink is a mixture of several coloured substances (or dyes) which can be separated by paper chromatography.
Notes
Matter in Our Surroundings - Notes
1. 11. Metals Usually Have a Silver or Grey Colour
Show Notes
2. The Case of a Liquid Solute Dissolved in a Liquid Solvent
Show Notes
8. 7. Non-Metals may be Solid, Liquid or Gases at the Room Temperature.
Show Notes
10. 3. Non-Metals are Bad Conductors of Heat and Electricity.
Show Notes
11. 6. Metals are Usually Strong. They Have High Tensile Strength.
Show Notes
17. Chemical Formula for daily use material
Show Notes
24. To Study the Properties of a Compound of Iron and Sulphur
Show Notes
28. 4. Metals are Lustrous (or Shiny), and can be Polished.
Show Notes
30. To Separate a Mixture of Alcohol and Water
Show Notes
31. To Study the Properties of a Solution
Show Notes
35. 7. Metals are Solids at the Room Temperature
Show Notes
36. To Study the Properties of a Suspension
Show Notes
39. To Separate the Salt-Water Mixture (or Salt-Solution)
Show Notes
46. Separation of Mixture Of Two Solids
Show Notes
48. 6. Non-Metals are Not Strong. They Have Low Tensile Strength.
Show Notes